Synthetic formation of a type of subcellular membraneless compartment in E. coli upon overexpression of disordered structural proteins such as spider silk and resilin.

This study has been 18 years in the making and is the longest study of my career. It’s a great example of the patience needed to see the science through from beginning to end.

Just like Internet of Things (IoT), IoB refers to access and control of human body via internet. Here, we detail how CRISPR can be used to electrically connect with the genome and as a proof of concept display control over transcriptional informational networks inside E. coli and Salmonella.

Synthetic biologists are increasingly using co-culture systems, but tools to control the composition of subpopulations are lacking. We designed a co-culture with signal translator and growth controller modules where culture composition autonomously adjusts in response to the AI-2 QS molecule.

Among the numerous SpCas9 variants that previously reported, we compared 13 variants to provide guidance to researchers. We developed a computational tool that predicts the activities of the variants to provide insights in selecting the most appropriate variants for their own research purposes.