We have designed a new method, Binding Affinities to Native Chromatin by sequencing (BANC-seq), to determine transcription factor concentrations needed to bind regulatory elements in the genome. We show that chromatin context and DNA accessibility are key regulators of transcription factor binding.

Functional studies of RNA pseudouridine (Ψ) modification have been limited by lacking quantitatively map individual Ψ sites transcriptome-wide. To address this challenge, Dai et al. developed BID-seq, a method for quantitative mapping of Ψ at single-nucleotide resolution.