Recent demonstration of immune responses to Cas proteins identify a critical risk in the eventual clinical application of Cas-mediated gene editing. In the accompanying paper we extend these findings by identifying promiscuous T-cell epitopes on Cas9 derived from Staphylococcus aureus.

M. Tyler Nelson & Mark R. Charbonneau--This article provides additional details on a successful collaboration between the 711th Human Performance Wing, Air Force Research Laboratory and Synlogic Inc., to develop human gut-on-a-chip models for screening high potential synbiotics.

We used proton gradients from top-down engineered light-harvesting E. coli to actuate DNA origami nanostructures. The DNA origami, in turn, controls the deformation of bottom-up assembled synthetic cells.