Nature Communications
An open access, multidisciplinary journal dedicated to publishing high-quality research in all areas of the biological, health, physical, chemical and Earth sciences.
A new CRISPR/Cas9 system with high editing efficiency and fidelity
We identified a novel type II-A nuclease, FrCas9. It possesses comparable cutting efficiency to SpCas9 but with lower off-target effect. Furthermore, the specific 5'-NNTA-3' PAM enables FrCas9 to directly target the TATA-box to regulate gene expression.
The Importance of False Discovery Rate Control in Metabolomics
We establish a novel approach to expand the utilization of FDR estimation by providing an automated false-discovery-rate-controlled analysis for data-independent acquisition in metabolomics.
