CRISPR arrays have proven difficult to generate due to the repetitive nature of their repeats. We developed a new method for assembly CRISPR arrays called CRATES, and we gained unique insights into CRISPR RNA biogenesis along the way.

The safety and security of medical devices driven by software, the software-development processes, and the need for data collection and privacy, all offer challenges and opportunities for device regulation and clinical care.

by Agnès E. Ricroch, Jacqueline Martin-Laffon & Marcel Kuntz. Nature Biotechnology. VOL 37. JUNE 2019. 601–621, DOI 10.1038/s41587-019-0138-7

Despite the improvement in trauma management and operative procedures, traumatic rupture of the thoracic aorta and cardiovascular penetrating injuries continue to be a source of significant mortality1. Currently, surgical suture is the only clinical method for aortic rupture and heart wound sealing but this is not feasible outside surgical units and not attempted in most emergency situations2. Though many experimental chemical agents have been tested for rapid wound sealing, such as fibrin glue, gelatin and collagen3-4. However, none of these materials are suitable for aortic and heart trauma hemostasis and sealing because of their slow hemostatic performance, poor wet tissue surface adhesion and weak or inflexible bonding mechanics.