Micropatterned microfluidics: dendronized fluorosurfactants for highly stable emulsions
We design surfactants that form robust microdroplets with improved high stability (in PCR thermal cycling) and resistance to inter-droplet transfer (of fluorescent dye salts, drugs, etc) using dendritic oligo-glycerol-based surfactants with a high degree of inter-and intramolecular hydrogen bonding.
Fluorosurfactant-stabilized microfluidic droplets are widely used as pico- to nanoliter volume reactors in chemistry and biology. However, current surfactants cannot completely prevent the inter-droplet transfer of small organic molecules encapsulated or produced inside the droplets. In addition, the microdroplets typically coalesce at temperatures higher than 80 °C. Therefore, the use of droplet-based platforms for ultrahigh-throughput combination drug screening and polymerase chain reaction (PCR)-based rare mutation detection has been limited. Here, we provide insights into designing surfactants that form robust microdroplets with improved stability and resistance to inter-droplet transfer. We used a panel of dendritic oligo-glycerol-based surfactants to demonstrate that a high degree of inter-and intramolecular hydrogen bonding, as well as the dendritic architecture, contribute to high droplet stability in PCR thermal cycling and minimize the inter-droplet transfer of the water-soluble fluorescent dye sodium fluorescein salt and the drug doxycycline. (Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules. Nat Commun 10, 4546 (2019). https://doi.org/10.1038/s41467-019-12462-5)
Related reading:https://orcid.org/0000-0001-7114-1095 https://www.nature.com/articles/s41467-019-12462-5 https://www.nature.com/articles/s41578-020-00247-y https://www.nature.com/articles/s41467-021-21436-5 https://bioengineeringcommunity.nature.com/posts/nature-derived-2-dimensional-materials-for-cancer-therapy-and-sustainable-solutions https://bioengineeringcommunity.nature.com/posts/tackling-covid-19-with-materials-science
Related micropatterned microfluidics studies:
Imaging systems and microfluidic devices for the in-depth and real-time investigation of viral structures and transmission，material platforms for organoids and organs-on-a-chip, in drug delivery and vaccination, and for the production of medical equipment.
- Z Tang†, N Kong†, X Zhang†, Y Liu, P Hu, S Mou, P Liljeström, J Shi, W Tan, J S Kim, Y Cao, R Langer, K W. Leong, O C. Farokhzad, W Tao. A materials-science perspective on tackling COVID-19. Nature Reviews Materials, 2020, 5, 847-860. (Featured Cover Paper, highly cited paper, accessed>19,000 times, Impact Factor:71.189)
Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules
- M Chowdhury, W Zheng, S Kumari, J Heyman, X Zhang, P Dey, D Weitz, R Haag. Dendronized fluorosurfactants provide phenomenal droplet integrity to picolitre emulsions for therapeutics development. Nature Communications, 2019, 10, 4546 (Impact Factor:14.919).
An osmotic-pressure, pH, excretion, nutrition, gas, ionic-strength, flow-rate, and temperature (OPEN GIFT) microenvironment-controlled micropatterned microfluidic model (MMMM) for biomimetic in situ studies (BISS) in simulating the in vivo microenvironment to study in situ the stress applied to Giardia in the intestinal tract.
- Y Wang, L Lu, G Zheng*, X Zhang*. Microenvironment-controlled micropatterned microfluidic model for biomimetic in-situ studies. ACS Nano, 2020, 14(8), 9861-9872. (Featured Cover Paper, Impact Factor:15.881).
- S Han†, Q Zhang†, X Zhang†, X Liu, L Lu, J Wei, Y Li, Y Wang, G A digital microfluidic diluter- based microalgal motion biosensor for marine pollution monitoring. Biosensors and Bioelectronics, 2019, 143, 111957 (Impact Factor:10.257).
- L Liu, N Xiang, Z Ni, X Huang, J Zheng, Y Wang, X Zhang*. Step Emulsification: High throughput production of monodisperse droplets. BioTechniques, 2020, 68 (3), 114-116. (Invited Expert Paper)
- S Wang, Z Shen, Z Shen, Y Dong, Y Li, Y Cao, Y Zhang, S Guo, J Shuai, Y Yang, C Lin, M Guo, X Chen*, X Zhang*, Q Huang*. Machine-learning micropattern manufacturing. Nano Today, 2021, 38 (2021), 101152. (Impact Factor:20.722)
- Z Li†, X Zhang† J Ouyang, D Chu, F Han, L Shi, R Liu, Z Guo, G Gu, W Tao, L Jin, J Li. Ca2+-supplying black phosphorus-based scaffolds developed with microfluidic technology for osteogenesis. Bioactive materials, 2021, 6(11), 4053-4064. (Instant Impact Factor:14.093).
Microfluidic technology for Theranostics.
- J Yang†, X Zhang†, C Liu†, Z Wang, L Deng, C Feng, W Tao, X Xua, W Cui. Biologically Modified Nanoparticles as Theranostic Bionanomaterials. Progress in Materials Science, 2021, 118, 100768. (Impact Factor:39.58)
- M Chowdhury†, X Zhang†, L Amini, A Faghani, A Singh, M Henneresse, R Haag. Functional Surfactants for Molecular Fishing, Capsule Creation, and Single-Cell Gene Expression. Nano-Micro Letters, 2021, 13, 147. (Impact Factor:16.419)
1. G Zheng, Q Gao, Y Jiang, L Lu, J Li，X Zhang, H Zhao, P Fan, Y Cui, F Gu, Y Wang. Instrumentation-compact digital microfluidic (DMF) reaction interface extended loop-mediated isothermal amplification (LAMP) for sample-to-answer testing of Vibrio parahaemolyticus. Analytical Chemistry, 2021.
- Zhang, Q., Zhang, X., Zhang, X., Jiang, L., Yin, J., Zhang, P., Han, S., Wang, Y.& Zheng, G. A feedback-controlling digital microfluidic fluorimetric sensor device for simple and rapid detection of mercury (II) in costal seawater. Marine pollution bulletin, 2019, 144, 20-27. https://doi.org/10.1016/j.marpolbul.2019.04.063
- R Yang, Z Gong, X Zhang, L Que. Single-walled carbon nanotubes (SWCNTs) and poly(3,4- ethylenedioxythiophene) nanocomposite microwire-based electronic biosensor fabricated by microlithography and layer-by-layer nanoassembly. Journal of Nanoscience and Nanotechnology, 2019,19(12), 7591-7595. https://doi.org/10.1166/jnn.2019.16752
Dr. Xingcai Zhang, Harvard/MIT Research Fellow; Science Writer/Editorial (Advisory) Board Member for Springer Nature, Elsevier, Materials Today, Royal Society of Chemistry, Wiley; Nature Nano Ambassador with 5 STEM degrees/strong background in sustainable Nature-derived/inspired/mimetic materials for biomed/sensing/catalysis/energy/environment applications, with around 100 high-impact journal publications in Nature Reviews Materials (featured cover paper), etc. orcid.org/0000-0001-7114-1095 https://scholar.google.com/citations?hl=en&user=2vDraMoAAAAJ&view_op=list_works&sortby=pubdate