Rapid cloning of antigen-specific T-cell receptors by leveraging the cis activation of T cells

Hi. Our article “Rapid cloning of antigen-specific T-cell receptors by leveraging the cis activation of T cells” has been published online in Nature Biomedical Engineering. Our lab is good at obtaining antigen-specific receptors of B and T lymphocytes at single cell levels.

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Previously we developed microwell array chips with 45,000 to 200,000 micro-wells that can capture single lymphocyte in it. By using them we can detect antigen-specific antibody-secreting cells at single cell levels, from which we produce antigen-specific antibodies. We call the system immunospot array assay on a chip (ISAAC). We then would like to use microwell array chips to detect antigen-specific T cells. Stimulation of T cells on a microwell array chip with antigen-presenting cells was difficult. We thought if single T cells can be activated by the interaction of T cell receptors (TCR) on a T cell and complex of antigenic peptide and MHC molecules (pMHC) on the same T cells and found we could it. We call the system “T-ISAAC” and the interaction of TCR and pMHC on the same T cell “cis-interaction”. In the article we showed that we can detect antigen-specific T cells with T-ISAAC and obtain antigen-specific TCRs.

Hiroyuki Kishi

Professor, University of Toyama